Journal: Virology Journal

Article Title: The recombinant E rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

doi: 10.1186/s12985-022-01851-w

Figure Lengend Snippet: Expression and identification of CSFV E rns and BVDV tE2 proteins. A & C The recombinant CSFV E rns ( A ) or BVDV tE2 ( C ) protein expressed in E. Coli was analyzed by SDS-PAGE, with Coomassie blue staining. M, molecular marker; Lane 1, lysate of bacteria harboring the control plasmid pET-28a; Lane 2, the lysate of bacteria harboring the plasmid pET-E rns ( A ) or pET-tE2 ( C ); Lane 3, the purified CSFV E rns ( A ) or BVDV tE2 ( C ) protein. B & D Recombinant CSFV E rns ( B ) or BVDV tE2 ( D ) protein was confirmed by western blotting using an anti-His monoclonal antibody (upper) or a specific anti-virus polyclonal antibody (lower). M, molecular marker; Lane 1, lysate of bacteria harboring the control plasmid pET-28a; Lane 2, the lysate of bacteria harboring the plasmid pET-E rns ( B ) or pET-tE2 ( D )

Article Snippet: To construct the expression plasmids, the codon-optimized E rns gene of CSFV Shimen strain and the coding sequence of truncated E2 (tE2, residues 690—865) of BVDV Hubei strain were synthetically produced (Sangon Biotech, Shanghai, China).

Techniques: Expressing, Recombinant, SDS Page, Staining, Marker, Bacteria, Control, Plasmid Preparation, Purification, Western Blot, Virus

Journal: Virology Journal

Article Title: The recombinant E rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

doi: 10.1186/s12985-022-01851-w

Figure Lengend Snippet: Optimizations of CSFV E rns and BVDV tE2 -based ELISA procedures. A & C Optimization of the concentration of coating antigen and the dilution of swine sera for CSFV E rns ( A ) or BVDV tE2 ( C ) -based ELISA using checkerboard titration test. B & D Optimization of the dilution of the secondary antibody for CSFV E rns ( B ) or BVDV tE2 ( D ) -based ELISA. P/N, positive control/negative control; ☆, the optimized condition for ELISA test

Article Snippet: To construct the expression plasmids, the codon-optimized E rns gene of CSFV Shimen strain and the coding sequence of truncated E2 (tE2, residues 690—865) of BVDV Hubei strain were synthetically produced (Sangon Biotech, Shanghai, China).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Titration, Positive Control, Negative Control

Journal: Virology Journal

Article Title: The recombinant E rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

doi: 10.1186/s12985-022-01851-w

Figure Lengend Snippet: Validation of the specificity of CSFV E rns and BVDV tE2 -based indirect ELISAs. The specificities of CSFV E rns -based ( A , C & E ) and BVDV tE2 -based ELISAs ( B , D & F ) were validated using a panel of infected swine sera ( A & B ), including PCV2 (n = 9), PRRSV (n = 6), PEDV (n = 4), ASFV (n = 5), CSFV (n = 10), BVDV (n = 10) and negative control (n = 6); a panel of immunized rabbit sera (C & D), including CSFV (n = 4), BVDV (n = 4) and negative control (n = 4) or immunized mouse sera ( E & F ), including CSFV (n = 6), BVDV (n = 6) and negative control (n = 6)

Article Snippet: To construct the expression plasmids, the codon-optimized E rns gene of CSFV Shimen strain and the coding sequence of truncated E2 (tE2, residues 690—865) of BVDV Hubei strain were synthetically produced (Sangon Biotech, Shanghai, China).

Techniques: Biomarker Discovery, Infection, Negative Control

Journal: Virology Journal

Article Title: The recombinant E rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

doi: 10.1186/s12985-022-01851-w

Figure Lengend Snippet: The sensitivity of CSFV E rns and BVDV tE2 -based ELISAs. A Evaluation of the sensitivity of CSFV E rns -based ELISA using serially swine sera with different neutralizing antibody (NAb) titers. P1, P2 and P3, anti-CSFV positive sera; N1, CSFV-free serum. The number in brackets indicated the NAb titer. B Evaluation of the sensitivity of BVDV tE2 -based ELISA using serially swine sera with different NAb titers. P1, P2 and P3, anti-BVDV positive sera; N1, BVDV-free negative serum. The number in brackets indicated the NAb titer

Article Snippet: To construct the expression plasmids, the codon-optimized E rns gene of CSFV Shimen strain and the coding sequence of truncated E2 (tE2, residues 690—865) of BVDV Hubei strain were synthetically produced (Sangon Biotech, Shanghai, China).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Virology Journal

Article Title: The recombinant E rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

doi: 10.1186/s12985-022-01851-w

Figure Lengend Snippet: Comparison of the BVDV tE2 -based indirect ELISA with virus neutralization test for detection of clinical serum samples of pigs

Article Snippet: To construct the expression plasmids, the codon-optimized E rns gene of CSFV Shimen strain and the coding sequence of truncated E2 (tE2, residues 690—865) of BVDV Hubei strain were synthetically produced (Sangon Biotech, Shanghai, China).

Techniques: Comparison, Indirect ELISA, Virus, Neutralization, Enzyme-linked Immunosorbent Assay